《植物生理学报》 2013, 49(11): 1250-1254

‘金州报春’兜兰的非共生萌发与快速繁殖

周丽^1,*, 邓克云², 徐正海²

¹兴义民族师范学院, 贵州兴义562400; ²黔西南州绿缘动植物科技开发有限公司, 贵州兴义562400

通信作者：周丽；E-mail: zhoulizxx@aliyun.com；Tel: 189085-99226

摘　要：

收集授粉后不同时期报春兜兰栽培品种‘金州报春’的种子, 观察种子形态特征和萌发过程, 建立了高效快繁体系。试验结果表明种子萌发的最佳培养基为RE+0.3 mg·L^-1 NAA +0.1mg·L^-1 6-BA +100 mL·L^-1 椰子乳(体积比); 授粉162 d后的种子萌发率较高并且无白化现象, 授粉203 d的种子播种后萌发率可达94.33%; 分化后的原球茎在壮苗培养基上培养4个月后可得到有5~6片叶、高3~5 cm的健壮试管苗。

关键词：种子; 非共生萌发; 培养基; 白化苗

收稿：2013-07-01   修定：2013-08-01

资助：贵州省科技厅项目[黔科合SY字(2012) 3030号]和贵州省教育厅项目[2011 (278)号]。

In vitro Asymbiotic Germination and Propagation of Paphiopedilum primulinum 'Jinzhoubaochun'

ZHOU Li1,*, DENG Ke-Yun2, XU Zheng-Hai2

¹Xingyi Normal University for Nationalities, Xingyi, Guizhou 562400, China; ²Southwest Guizhou Luyuan Animal and Plant Technologies Company Limited, Xingyi, Guizhou 562400, China

Corresponding author: ZHOU Li; E-mail: zhoulizxx@aliyun.com; Tel: 189085-99226

Abstract:

In this experiment, we collected different embryo-aged-seeds of Paphiopedilum primulinum ‘Jinzhoubaochun’, observed the structure and the process of seed germination, and established an effective regeneration system. The results showed that the better medium for germination was Robert Ernest (RE)-based media containing 100 mL·L^-1coconut water, 0.3 mg·L^-1 NAA and 0.1 mg·L^-1 6-BA. 162 DAP (day after pollination) seeds had higher germination rate and without albino seedling, germination rate of 203 DAP seeds were 94.33%. Transferred differentiation protocorns on optimum rooting medium, after 4 months, the healthy robust seedlings were 3–5 cm tall and had 5–6 leaves.

Key words: seed; asymbiotic germination; medium; albino seedling